rabbit anti ppar gamma polyclonal antibody  (Bioss)

Journal: Foods

Article Title: Citri grandis Exocarpium Extract Alleviates Atherosclerosis in ApoE −/− Mice by Modulating the Expression of TGF-β1, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1

doi: 10.3390/foods14244267

Figure Lengend Snippet: Representative blotting images and protein densitometry showing the relative expression levels of TGF-β, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1 proteins normalized with GAPDH and β-actin in aorta of apoE −/− mice . ( A , E ) are the representative protein blotting images; ( B – D , F – H ) Expression levels of TGF-β, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1 proteins in aorta were shown in the bar chart and the blotting grayscale value analyses normalized with GAPDH and β-actin (Wilks’ Λ = 0.003, R 2 = 0.970, Eta 2 = 0.970), which were used as loading control. The error bars represented x ¯ ± SE. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the MOD group indicate a statistically significant difference; n = at least 3 for each group.

Article Snippet: Polyclonal antibodies for AKT1 (rabbit), PPAR-γ (rabbit), LXR-α (rabbit), and ABCA1 (rabbit) were obtained from Proteintech (Wuhan, China).

Techniques: Expressing, Control

Journal: Foods

Article Title: Citri grandis Exocarpium Extract Alleviates Atherosclerosis in ApoE −/− Mice by Modulating the Expression of TGF-β1, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1

doi: 10.3390/foods14244267

Figure Lengend Snippet: Effect of CGE on the expression of TGF-β, PI3K, AKT, PPAR-γ, LXR-α, and ABCA1 in mouse livers . ( A – F ) represent the mRNA expression levels of TGF-β, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1 to GAPDH in livers, respectively (Wilks’ Λ = 0.001, R 2 = 0.953, Eta 2 = 0.958). ( G , K ) are the representative protein blotting images; ( H – J , L – N ) are the respective optical density ratios of bands, which represent the relative expression levels of TGF-β, PI3K, AKT, PPAR-γ, LXR-α, and ABCA1 proteins normalized with GAPDH or β-actin in the livers of apoE −/− mice (Wilks’ Λ = 0.008, R 2 = 0.986, Eta 2 = 0.986). The blotting grayscale value was analyzed relative to the GAPDH band and β-actin, as a loading control. The error bars represent the x ¯ ± SE. It is considered to have statistically significant differences when * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the MOD group; n = at least 3 for each group.

Article Snippet: Polyclonal antibodies for AKT1 (rabbit), PPAR-γ (rabbit), LXR-α (rabbit), and ABCA1 (rabbit) were obtained from Proteintech (Wuhan, China).

Techniques: Expressing, Control